Purification of cortactin using anion exchange chromatography (755.2)
نویسندگان
چکیده
منابع مشابه
Purification of DNA by anion-exchange chromatography.
Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...
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Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...
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Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
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Background Prothrombin complex contains the vitamin K dependent coagulation factors II, VII, IX, X, protein C, and protein S. It has been used for the treatment of congenital coagulation disorders and is recommended for reversing oral anticoagulation [1]. Prothrombin complex proteins require Ca to express their activities. The conformational change induced by Ca finds a practical application in...
متن کاملoptimization of dynamic binding capacity of anion exchange chromatography media for recombinant erythropoietin purification
background:the dynamic binding capacity (dbc) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. optimization of the process criteria for maximum dbc avoids extra process scale-up and reduces the processing time, costs and protein loss. taguchi method is a simple usef...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2014
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.28.1_supplement.755.2